Quantitative phase microscopy – principles, instrumentation and biomedical applications

What to expect

Label-free cell studies are based on intrinsic refractive index changes within cells or tissues, directly related to the morphology of these samples. This type of measurements allows to study objects unchanged by the fluorescent dyes, which shows prospect of e.g. reintroducing cells back into donor.

The aim of the training is to provide access and expertise to potential users of label-free phase microscopy in techniques such as Digital Holographic Microscope with focus on phase/dry mass measurement and monitoring in biological cells and cell cultures, Fourier Ptychographic Microscopy with focus on amplitude and phase imaging of histopathological samples and Optical Diffraction Tomography for 3D QPI of refractive index distribution in cells/cell cultures/tissues.

The participants will have unique chance to work on state-of-the-art measurement instruments -either custom-made or commercially-available, applied to biological objects’ studies.

This unique industry ‘hands-on’ training program includes dedicated tutorials, tutorial material, and mentoring by technical experts.

Course outcomes

• Understanding of key features of quantitative phase imaging for biomedical applications.
• Understand the design of 2D and 3D phase imaging microscopic systems based on coherent and incoherent light (hands-on activity).
• Understand the full data processing scheme and its influence on the proper interpretation of results (hands-on activity).
• Understand the influence of biological sample preparation procedure on measurement result.
• Understand & be able to determine the metrological parameters of QPI devices (hands-on activity).

Course audience

Attendees from biomedical sectors such as diagnosis/histopathological medical laboratories/hospitals, cell and tissues factories, pharmaceutical industry

Attendee experience

Some previous experience would be desirable but not essential